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Tuesday, July 21, 2020 | History

2 edition of Polarized secretion of IL-6 by intestinal epithelial cells found in the catalog.

Polarized secretion of IL-6 by intestinal epithelial cells

John Omar Mascarenhas

Polarized secretion of IL-6 by intestinal epithelial cells

by John Omar Mascarenhas

  • 150 Want to read
  • 9 Currently reading

Published .
Written in English

    Subjects:
  • Epithelial cells.

  • Edition Notes

    Statementby John Omar Mascarenhas.
    Series[Honors theses. Science and mathematics / State University of New York at Binghamton -- v. 146], Honors theses -- v. 146.
    The Physical Object
    Pagination39, [9] leaves :
    Number of Pages39
    ID Numbers
    Open LibraryOL22199654M

      Stimulation of IL-6 by other stimuli is polarized. To see if IL-6 secretion is polarized after stimulation by other proinflammatory stimuli, T84 cells were stimulated with S. typhimurium and TNF-α (both of which have been shown to induce IL-6 transcripts in intestinal epithelial cells) and forskolin, a direct activator of adenylate cyclase. Stimulation of T84 monolayers with either apical or basolateral adenosine induces A2b receptor–mediated increase in IL-6 secretion, which is polarized to the apical (luminal) compartment. In addition, Salmonella typhimurium, TNF-α, and forskolin, known inducers of IL-6 secretion in intestinal epithelial cells, also stimulate IL-6 secretion.

    The polarized delivery of membrane proteins to the cell surface and the initial secretion of lysosomal proteins into the culture medium were studied in the polarized human intestinal adenocarcinoma cell line Caco-2 in the presence or absence of the microtubule-active drug nocodazole.   A complex physical barrier composed by a monolayer of polarized epithelial cells has been of intestinal inflammation, IL secretion by APC and T cells but not IL-6, is critical for the.

    Intestinal epithelial cells are columnar epithelial cells that form a barrier in the gastrointestinal epithelium. These polarized cells possess specific structures such as microvilli on their apical surface and play important roles in nutrient absorption and in protecting against infection by .   Introduction. Intestinal barrier dysfunction mainly refers to the abnormal increased intestinal permeability, which can make pathogens and foreign antigens cross the epithelial barrier[].Intestinal permeability is regulated by tight junctions(TJ) formed between intestinal epithelial cells (IEC) at the most apical areas of the epithelium.


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Polarized secretion of IL-6 by intestinal epithelial cells by John Omar Mascarenhas Download PDF EPUB FB2

Polarized secretion of IL-6 has been reported for mouse uterine epithelial cells. Polarized secretion of the inflammatory cytokines IL-6 and IL-8 was investigated with monolayers of human RPE cells on transwell filters. RPE cells were stimulated at the apical or the basal side with IL-1β and the secretion of IL-6 and IL-8 was by: Intestinal epithelial cells (IEC) can exist as polarized cells and are capable of secreting interleukin-6 (IL-6), yet it has not been determined if this IL-6 is secreted in a polarized by: The ARPE‐19 cell line also secreted IL‐6 and IL‐8 in a polarized fashion towards the basal side after basal stimulation; polarized secretion was, however, not apparent after apical stimulation.

The observation that human RPE cells secrete IL‐6 Polarized secretion of IL-6 by intestinal epithelial cells book IL‐8 in a polarized fashion towards the choroid may represent a mechanism to prevent Cited by:   Stimulation of IL-6 by other stimuli is polarized.

To see if IL-6 secretion is polarized after stimulation by other proinflammatory stimuli, T84 cells were stimulated with S. typhimurium and TNF-α (both of which have been shown to induce IL-6 transcripts in intestinal epithelial cells) and forskolin, a direct activator of adenylate by:   In summary, the cationic polypeptide, poly-l-arginine, induces a time- and concentration-dependent polarized IL-6 and IL-8 secretion towards the apical side of bronchial epithelial cells, effects that are mediated via p, ERK1/2- and NF-κB-dependent pathways.

This phenomenon may contribute to the pathophysiology of asthmatic inflammation Cited by: The direction of cytokine secretion from polarized cells determines the cytokine's cellular targets.

Leukemia inhibitory factor (LIF) belongs to the interleukin-6 (IL-6) family of cytokines and signals through LIFR/gp Three factors which may regulate the direction of LIF secretion were studied: the site of stimulation, signal peptides, and expression levels. Directly beneath the epithelial cell layer resides the body’s largest pool of immune cells, which maintain intestinal homeostasis via removal of invading bacteria and dying cells, secretion of anti-inflammatory cytokines, and induction/maintenance of tolerance.

The intestinal barrier is established by a single layer of polarized intestinal epithelial cells (IECs) separating bacteria in the intestinal lumen from the immune cells in the lamina propria.

IECs in the intestinal crypt form a rapidly proliferating cell population which completely replaces the epithelium with newly generated cells over a. Infection of intestinal epithelial cells with the F4 + ETEC reference strain GIS26 significantly induced the apical and basolateral secretion of both IL-6 (Fig.

4A) and IL-8 (Fig. 4B) as compared to non-infected cells and cells infected with the non-pathogenic E. coli strain HB   The function of cell adhesion is dependent on three factors: (1)presence of cadherins (adhesive glycoproteins) in the lateral plasma membrane of the epithelial cells, (2) presence of invaginations and evaginations in the lateral plasma membranes, due to which the cells presence of adhering and occluding cell junctions between the adjacent cells.

The intestinal epithelium is a highly organized tissue. The establishment of epithelial cell polarity, with distinct apical and basolateral plasma membrane domains, is pivotal for both barrier formation and for the uptake and vectorial transport of nutrients.

The establishment of cell polarity requires a specialized subcellular machinery to transport and recycle proteins to their appropriate. The polarized secretion of a number of cytokines including IL-6 has been described in Caco-2 cells.

Secretion of LIF by Caco-2 has not been previously reported. Caco-2 cells were grown as a confluent monolayer in tissue culture flasks for two weeks to allow them to fully differentiate.

Cell polarity is a fundamental feature of many types of lial cells are one example of a polarized cell type, featuring distinct 'apical', 'lateral' and 'basal' plasma membrane domains. Epithelial cells connect to one another via their lateral membranes to form epithelial sheets that line cavities and surfaces throughout the animal body.

Each plasma membrane domain has a distinct. A schematic presentation of non-polarised and polarized intestinal cell epithelial cells under non-infective conditions or during infection with (A) Cultured non-polarised intestinal epithelial cells such as INT do not express typical cell-to cell junctions.

Thus, basolateral receptors such as focal adhesion structures are accessible and not protected by tight or adherens junctions. Epithelial barrier function after co-culture of intestinal epithelial cells (IECs) with monocytes derived from celiac disease patients. After peripheral blood mononuclear cell (PBMC) isolation and CD14+ cell-sorting, epithelial cells were co-cultured with monocytes from healthy donors.

celiac patients on a gluten-free diet (GFD), or AC (active celiac disease (CeD)) patients. Polarized cells establish and maintain functionally distinct surface domains by an elaborate sorting process, which ensures accurate delivery of biosynthetic cargo to different parts of the plasma membrane.

This is particularly evident in polarized epithelial cells, which have been used as a model system for studies of sorting mechanisms. The clustering of lipid rafts through the. Gills Epithelial Cells Wood Thymus Gland Branchial Region Endoderm Mesoderm Muscle, Skeletal Ribosome Subunits, Small.

Organisms 6. Oncorhynchus mykiss Trout Fishes Dictyocaulus Mytilidae Bivalvia. Diseases 2. Hypercapnia Dictyocaulus Infections. Chemicals and Drugs Secretion Epithelia serve a number of functions. They o!er protection to organs such as the skin. An example of the importance of proper sorting of proteins to the apical and basal surfaces is the intestinal epithelial cell.

One function of these cells is to Microtubules are polarized in epithelial cells with their minus ends at apical. A glandular epithelium contains many secretory cells. The Epithelial Cell. Epithelial cells are typically characterized by the polarized distribution of organelles and membrane-bound proteins between their basal and apical surfaces.

Particular structures found in some epithelial cells are an adaptation to specific functions. Viral infection from the basolateral side of intestinal epithelial cells (IECs) is shown to elicit a stronger intrinsic immune response than apical infection, an effect driven by the polarized.

The epithelial cells that cover each small intestinal villus are derived from progenitors (i.e., stem cells) within the crypt (Potten and Loeffler, ; Barker et al., ). Relatively undifferentiated, secretory epithelial cells exit the crypt base and proliferate within a region termed the transit amplifying zone.Abstract.

Intestinal epithelial cells (IEC) are known to secrete a variety of important cytokines including the inflammatory cytokine interleukin-6 (IL-6).

1,2 Along with its role in the inflammatory response, IL-6 has also been shown to act as a co-stimulator for T cell proliferative responses 3 and is known to induce Peyer’s patch and appendix B cells to secrete high levels of IgA.

4, 5.3. Transport Studies in Other Epithelial Cell Lines. A number of other cultured intestinal cell lines are commercially available for studies of epithelial physiology (HT29 cells, Caco2 cells). The parent HT29 cell line is unpolarized, but several polarized subclones have been derived that are useful for studies of epithelial secretion.